An in vitro Multi-Enzyme Cascade for Efficient One-pot Biosesynthesis of Sorbitol from Fructose-6-Phosphate
Sorbitol is a naturally occurring sugar alcohol with diverse biological properties, which is widely applied in the food, cosmetic, and pharmaceutical industries.
Although a biosesynthetic pathway from starch to fructose-6-phosphate (F-6-P) has been well established in metabolic engineering studies, the downstream enzymatic route leading to the synthesis of sorbitol from F-6-P has not yet been elucidated in the literature.
In this study, the authors performed the design and implementation of an artificial multi-enzymatic cascade system for the biosesynthesis of sorbitol from fructose-6-phosphate.
Following systematic optimization of this multi-enzyme cascade system, 82.6 mM of sorbitol was efficiently produced from 200 mM fructose-6-phosphate.
